ocn  (Novus Biologicals)

Journal: bioRxiv

Article Title: Interleukin-3 as a Potential Bone Anabolic Agent in treating Postmenopausal Osteoporosis

doi: 10.1101/2025.07.12.664485

Figure Lengend Snippet: IL-3 alters OVX-induced changes in osteoclasts- and osteoblasts-specific gene expression. Marrow free bones from IL-3 treated animals from both Prophylactic and Therapeutic treatment strategy, as indicated, were subjected to mRNA expression profile and ( A, B ) Osteoclast-specific markers: integrinβ3, Dc-stamp, and Nfatc1 , Ctsk, Tnfrsf11a(RANK), Mmp9, Trap, and Ctr. (B, D) Osteoblast-specific markers: Runx2, Osx, Opn, Alp, Bmp2, Bmp4, Ocn, Bmp6, and Col1 were assessed by qPCR. Serum ( E, G ) CTX and ( F, H ) OCN were also measured by ELISA in both the strategies. Data is presented as mean ± SEM with n=3 mice/group for qPCR, and n=8 mice/group for ELISA.

Article Snippet: The serum level of CTX-I (CUSABIO; CSB-E12782m) and OCN (CUSABIO ; CSB-E06917m) was assessed by ELISA according to manufacturers’ instructions.

Techniques: Gene Expression, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Food Science & Nutrition

Article Title: Luteolin Inhibits Dexamethasone‐Induced Osteoporosis by Autophagy Activation Through miR‐125b‐5p/SIRT3/AMPK/mTOR Axis, an In Vitro and In Vivo Study

doi: 10.1002/fsn3.70071

Figure Lengend Snippet: Effects of DEX and LUT on BMSC osteogenesis, miR‐125b‐5p expression, and SIRT3/AMPK/mTOR axis. The administration of LUT restored the levels of RUNX2 (a), OSX (b), OPN (c), and OCN (d). Moreover, altered levels of miR‐125b‐5p expression (e), as well as gene expression and protein levels of SIRT3 (f, i), AMPK (g, j), and mTOR (h, k) were obtained after DEX and LUT treatment. p ‐value < 0.05 was considered significant.

Article Snippet: ELISA kits were purchased for the measurement of SIRT3 (Catalogue number: abx156091, abbexa, UK), AMPK (Catalogue number: NBP3‐42385, Novus Biologicals, USA), mTOR (Catalogue number: RTFI01278, AssayGenie, Ireland), Runx2 (Catalogue number: abx255979, abbexa, UK), OSX (Catalogue number: MBS020109, MyBioSource, USA), OPN (Catalogue number: KA0312, Novus Biologicals, USA), OCN (Catalogue number: NBP2‐68153, Novus Biologicals, USA), LC3 (Catalogue number: RTEB1503, AssayGenie, Ireland), ATG5 (Catalogue number: RTEB1055, AssayGenie, Ireland), BECN1 (Catalogue number: NBP2‐69960, Novus Biologicals, USA), and P62 (Catalogue number: NBP2‐61300, Novus Biologicals, USA).

Techniques: Expressing, Gene Expression

Journal: Food Science & Nutrition

Article Title: Luteolin Inhibits Dexamethasone‐Induced Osteoporosis by Autophagy Activation Through miR‐125b‐5p/SIRT3/AMPK/mTOR Axis, an In Vitro and In Vivo Study

doi: 10.1002/fsn3.70071

Figure Lengend Snippet: Effects of DEX and LUT on osteogenesis markers. The administration of LUT restored the gene expression and protein levels of RUNX2 (a, e), OSX (b, f), OPN (c, g), and OCN (d, h). Similar lowercase letters on bars represent no significant difference between groups. p ‐value < 0.05 was considered significant.

Article Snippet: ELISA kits were purchased for the measurement of SIRT3 (Catalogue number: abx156091, abbexa, UK), AMPK (Catalogue number: NBP3‐42385, Novus Biologicals, USA), mTOR (Catalogue number: RTFI01278, AssayGenie, Ireland), Runx2 (Catalogue number: abx255979, abbexa, UK), OSX (Catalogue number: MBS020109, MyBioSource, USA), OPN (Catalogue number: KA0312, Novus Biologicals, USA), OCN (Catalogue number: NBP2‐68153, Novus Biologicals, USA), LC3 (Catalogue number: RTEB1503, AssayGenie, Ireland), ATG5 (Catalogue number: RTEB1055, AssayGenie, Ireland), BECN1 (Catalogue number: NBP2‐69960, Novus Biologicals, USA), and P62 (Catalogue number: NBP2‐61300, Novus Biologicals, USA).

Techniques: Gene Expression

Journal: Redox report : communications in free radical research

Article Title: METTL14 Mediates Glut3 m6A methylation to improve osteogenesis under oxidative stress condition.

doi: 10.1080/13510002.2024.2435241

Figure Lengend Snippet: Figure 4. METTL14 regulates H2O2-induced MC3T3-E1 cell viability, glycolysis, and osteogenic differentiation via GLUT3. MC3T3-E1 cells were transduced with Mettl14 overexpressing adenovirus (oeMettl14) alone or in combination transfected with Glut3 interfering siRNA (siGlut3) and then treated with 200 μM H2O2 for 12 h. (A) CCK-8 detected cell viability. (B) Seahorse Energy Analyzer detected ECAR. Biochemical assays detected (C) ATP content and (D) ALP activity. (E) Ali zarin Red S staining detected calcium deposition (scale bar: 50 μm). (F) Western blot detected COL1A1 and OCN expression. (G) qRT-PCR and (H) Western blot detected GLUT3 expression. Experiments were repeated independently three times and values are mean ± SD. *P<0.05, **P<0.01, ***P<0.001 vs. control; #P<0.05, ##P<0.01, ###P<0.001 vs. H2O2; ΔΔΔP<0.001 vs. H2O2+oeMettl14.

Article Snippet: The levels of C-telopeptide of type I collagen (CTX-I), Tartrateresistant alkaline phosphatase 5b (TRAP-5b), osteocalcin (OCN) and bone alkaline phosphatase (BALP) in serum were detected using CTX-I (E-EL-M3023), TRAP-5b (E-EL-M1116), OCN (E-EL-M0864; Elabscience Biotechnology, Inc.) and BALP (E-EL-M0200; all from Elabscience Biotechnology, Inc.) ELISA kits according to the manufacturers’ instructions.

Techniques: Transduction, Transfection, CCK-8 Assay, Activity Assay, Staining, Western Blot, Expressing, Quantitative RT-PCR, Control

Journal: Redox report : communications in free radical research

Article Title: METTL14 Mediates Glut3 m6A methylation to improve osteogenesis under oxidative stress condition.

doi: 10.1080/13510002.2024.2435241

Figure Lengend Snippet: Figure 5. Glut3 overexpression inhibits Mettl14 interference-induced MC3T3-E1 cell viability, glycolysis, and osteogenic differentiation. MC3T3-E1 cells were trans duced with Mettl14 interfering adenovirus (shMettl14) alone or in combination transfected with Glut3 overexpression plasmid (oeGlut3). (A) CCK-8 detected cell viability. (B) Seahorse Energy Analyzer detected ECAR. Biochemical assays detected (C) ATP content and (D) ALP activity. (E) Western blot detected COL1A1 and OCN expression. (F) qRT-PCR and (G) Western blot detected GLUT3 expression. Experiments were repeated independently three times and values are mean ± SD. *P<0.05, **P<0.01, ***P<0.001 vs. shNC; ###P<0.001 vs. shMettl14-1.

Article Snippet: The levels of C-telopeptide of type I collagen (CTX-I), Tartrateresistant alkaline phosphatase 5b (TRAP-5b), osteocalcin (OCN) and bone alkaline phosphatase (BALP) in serum were detected using CTX-I (E-EL-M3023), TRAP-5b (E-EL-M1116), OCN (E-EL-M0864; Elabscience Biotechnology, Inc.) and BALP (E-EL-M0200; all from Elabscience Biotechnology, Inc.) ELISA kits according to the manufacturers’ instructions.

Techniques: Over Expression, Transfection, Plasmid Preparation, CCK-8 Assay, Activity Assay, Western Blot, Expressing, Quantitative RT-PCR

Journal: Redox report : communications in free radical research

Article Title: METTL14 Mediates Glut3 m6A methylation to improve osteogenesis under oxidative stress condition.

doi: 10.1080/13510002.2024.2435241

Figure Lengend Snippet: Figure 6. Mettl14 overexpression inhibits bone loss in a mouse model of osteoporosis. An osteoporosis model was constructed by OVX in mice, and Mettl14 over expression adenovirus (oeMettl14) was intratibially injected for 8 weeks. (A) micro-CT analysis of tibial histomorphometric parameters, including (B) BV/TV, (C) Tb. Th, (D) Tb. N, (E) Tb. Sp, and (F) BMD. (G) H&E and Masson staining indicating the formation of new bone (scale bar: 100 μm). ELISA for serum (H) CTX-I, (I) TRAP-5b, (J) OCN, and (K) BALP. (L) qRT-PCR and (M) Western blot detected expression of METTL14 and GLUT3 in tibia. Experiments were repeated independently three times and values are mean ± SD. *P<0.05, **P<0.01, ***P<0.001 vs. sham; #P<0.05, ##P<0.01, ###P<0.001 vs. OVX+vector.

Article Snippet: The levels of C-telopeptide of type I collagen (CTX-I), Tartrateresistant alkaline phosphatase 5b (TRAP-5b), osteocalcin (OCN) and bone alkaline phosphatase (BALP) in serum were detected using CTX-I (E-EL-M3023), TRAP-5b (E-EL-M1116), OCN (E-EL-M0864; Elabscience Biotechnology, Inc.) and BALP (E-EL-M0200; all from Elabscience Biotechnology, Inc.) ELISA kits according to the manufacturers’ instructions.

Techniques: Over Expression, Construct, Injection, Micro-CT, Staining, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Western Blot, Expressing, Plasmid Preparation